Whitepaper
Enabling Therapeutic Progress with Oligonucleotide Bioanalysis
Analytical approaches for oligonucleotides in biofluids and tissues
Compare hELISA, qPCR and LC-MS/MS for oligonucleotide quantification, and explore BioSpring’s approach using isobaric internal standards.
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About this whitepaper
Reliable bioanalytical data support the evaluation of oligonucleotide therapeutics and their metabolites in biofluids and tissues. Chemical modifications, conjugations, degradation products and biological matrix effects can complicate quantification. This whitepaper discusses method selection for these challenges and introduces BioSpring’s LC-MS/MS approach using isobaric internal standards.
What you’ll learn
- Bioanalytical challenges in oligonucleotide development and their implications for method selection.
- Key quantification approaches: hybridization ELISA (hELISA), quantitative PCR (qPCR) and LC-MS/MS.
- BioSpring’s use of isobaric internal standards for LC-MS/MS quantification in biological matrices.
Who is it for?
Scientists and teams planning bioanalytical work for therapeutic oligonucleotides, including studies in biofluids and tissues.